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ACCURATE DATA
ANIMAL SAMPLES
CONTACTS
TRANSPORT
WATER SAMPLES

 

ACCURATE DATA

Background

  1. Record keeping is an essential component of effective aquatic animal health management.
  2. These can be included in routine records of an animal's growth that ideally would be monitored on a regular basis, either by sub-sampling from tanks or ponds, or by estimates made from surface observations.
  3. Where possible, hatcheries should invest in a microscope and conduct daily microscopic examinations of the larvae.
  4. This will allow them to quickly identify problems developing with their stocks, often before they become evident in the majority of the population.

Disease Awareness and Control

For hatcheries, critical information that should be recorded include:

  • Feeding activity
  • Growth (not daily, but regularly)
  • Mortalities (number and cause)
  • Dissolved oxygen
  • Water temperature

These observations should be recorded daily, for all stages, including date, time, tank number, broodstock (where there are more than one) and food source.

Dates and times of tank and water changes, pipe flushing/back-flushing and/or disinfections, should also be recorded.

Ideally, these records should be checked (signed off) regularly by the person responsible for maintaining the facility.

For pond or net/cage sites, observations that need to be recorded include:

  • Growth
  • Fouling
  • Mortalities
  • Dissolved oxygen
  • Water temperature

These should be recorded with date, site location and any relevant activities (e.g., sample collection for laboratory examination).

As elsewhere, the person responsible for the facility should check these records regularly.
The frequency of these observations will vary with site and fish species.

Human activities should also be recorded on an "as it happens" basis, since there may be time-lag effects. In all cases, date and time should be recorded, as parameters such as temperature and pH can vary markedly during the day, particularly in open ponds and inter-tidal sites.

All movements of fish into and out of a hatchery or site should be recorded, including:

  • The source of the broodstock/eggs/larvae/juveniles and their health certification
  • The volume or number of fish condition on arrival
  • Any quarantine measures carried out
  • Date and time of delivery and name of person responsible for receiving the fish
  • Date, time and destination of stock shipped out from a hatchery or site

Such records are also applicable (but less critical) to movements between tanks, ponds, and cages within a site.

Where possible, animals from different sources should not be mixed. If mixing is unavoidable, keep strict records of which sources are mixed and dates of new introductions into the holding site or system.

For molluscs, observations can be included with routine monitoring of mollusk growth, either by sub-sampling from suspension cages, lines or stakes, or by guess estimates from surface observations.

Environmental issues must also be monitored on a regular basis.

This is most applicable to open water sites, but should also be included in land-based systems with flow-through or well-based water sources.

The minimum essential data that should be recorded is:

  • Temperature
  • Salinity
  • Turbidity (qualitative evaluation or secchi disc)
  • Algal blooms…and
  • Human activity

The frequency of these observations will vary with site.

Where salinity or turbidity rarely vary, records may only be required during rainy seasons or exceptional weather conditions.

Temperate climates will require more frequent water temperature monitoring than tropical climates.

Human activity should be logged on an "as it happens" basis for reference if no infections or natural environmental changes can be attributed to a disease situation.

For Crustacean Hatchery operations, the minimum essential information that should be recorded/ logged includes:

  • Feeding activity and feed rates
  • Growth/larval staging
  • Mortalities…and
  • Larval condition

These observations should be recorded on a daily basis for all stages, and include date, time, tank, broodstock (where there are more than one) and food-source (e.g., brine shrimp culture batch or other food-source).

Dates and times for tank and water changes should also be noted, along with dates and times for pipe flushing and/or disinfections.

Ideally, the person responsible should check these logs regularly for the site/animals.


For pond sites, the minimum essential observations that need to be recorded/logged include:

  • Growth
  • Feed consumption
  • Fouling…and
  • Mortality

These should be recorded with date, site location and any action taken (e.g., sample collection for laboratory examination).

It is important to understand that rates of change for these parameters are essential for assessing the cause of any disease outbreak.

This means levels have to be logged on a regular and consistent basis in order to detect patterns over time.

Ideally, the person responsible for the site/animals should check these logs regularly.

If an outbreak does occur, the collection of accurate data that can be passed on to the lab, could mean the difference between a quick and effective diagnosis; and therefore a remedy; or a total loss of all animals!

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ANIMAL SAMPLES

Background

The best time to make observations about farm animals is at a time when there are no problems on the farm.

"Normal behaviour" of the animals should be observed from day one to establish and describe the "normal" situation.

Any change from this normal behaviour should be a cause for concern and warrants further investigation.

With finfish, abnormal behaviour includes fish swimming near the surface, sinking to the bottom, loss of balance, flashing, corkscrewing or air gulping (non air-breathers) or any sign which deviates from normal behaviour.

Bursts of abnormal activity are often associated with a generalised lethargy.

If losses persist or increase, samples should be sent for laboratory analysis.

Mortalities that are unusual, or cannot be explained should be examined immediately and environmental factors during, pre- and post-mortality recorded.

Some jurisdictions require farmers to report such mortalities.

It is difficult to observe behavioural changes in molluscs in open-water, however, close attention can be made of behaviour of both broodstock and larvae in hatcheries.

In Crustaceans such as prawns, this may be a subtle change in feeding behaviour, swimming movement or unusual aggregations.

Even predator activity can provide clues to more "hidden" changes such as when fish- or shrimp-eating birds congregate round affected ponds.

Since disease situations can erupt very quickly under hatchery conditions, regular and close monitoring is essential; for example, feeding behaviour of larval molluscs is a good indicator of general health.

Mortalities that spread from one area to another may suggest the presence of an infectious disease agent and should be sampled immediately.

Affected animals should be kept (isolated) as far away as possible from unaffected animals until the cause of the mortalities can be established.


Disease Awareness and Control
All samples submitted for diagnosis should include as much supporting information as possible including:

  • Contact details of submitter
  • Reason(s) for submitting the sample (i.e. health screening, certification) handling activities
  • Gross observations, feed records, and environmental parameters
  • History and origin of the animal population date of transfer and
  • Source location(s) if the stock does not originate from on-site.

A Contingency Plans will help clarify whether handling stress, change of environment or infectious agents are causes for concern.

It will also help speed up diagnosis, risk assessment, and husbandry management and treatment recommendations.

The most important factors associated with collection of specimens for surveillance are:

  • Sample numbers that are high enough
  • Susceptible species are sampled
  • Sampling includes age groups and seasons that are most likely to manifest detectable infections.

Environmental changes (rapid water quality changes, such as turbidity fluxes, saltwater incursion into freshwater ponds, unusual weather events, etc.) should also be noted and information on these events should be included with the specimens.

It's critical to establish, and record, normal behaviour and appearance to compare with observations made during disease events.

Accurate record keeping is, therefore, an essential component of effective aquatic animal health management.

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CONTACTS

Background

  1. Aquatic environments are complex ecosystems, making the distinction between health, suboptimal performance and disease obscure.
  2. During disease outbreaks, the underlying cause is often difficult to ascertain and is usually the end result of a series of linked events involving:
    • Environmental factors
    • Health condition of the stocks
    • Presence of an infectious agent and/or poor husbandry, and….
    • Management practices.
  3. The whole aquatic production environment, including ecological processes, must be taken into consideration when assessing whether disease is an issue.
  4. As in pastoral based livestock, the aquaculture and fisheries sector will continue to face increasing global exposure to disease agents as it intensifies trade in live aquatic organisms and their products.

Disease Awareness and Control
The first and most important defenses against preventable disease losses under such complex situations are:

  • Monitoring as regularly as possible and
  • Appropriate and decisive action at the first sign of suspicious behaviour, lesions or mortalities.

Some aquaculture farmers may hesitate to report the first signs of health problems, due to concern that it may reflect on their production capability, or that it will result in failure in the competitive market place. Hiding or denying health problems, however, can be as destructive to aquatic animals as it is in other agriculture endeavors.

It is important to recognise that disease is a challenge that everyone has to face, and having the resources that can effectively deal with it, are the primary weapons against misplaced ignorance and fear.

Every State Government has a representative that can be contacted if an exotic or other disease is suspected.

In Western Australia and in Tasmania the State Governments provide a diagnostic framework and encourage farmers to make them the first port of call.

There are also local vets, commercial laboratories and specialists at many of the TAFE and University based training Institutions, and of course AFFA from the Australian Government.

The main thing is to act responsibly…and quickly!

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TRANSPORT

  1. Shipping samples for diagnosis is as important as preparing the samples themselves.
  2. In the case of live animals, it's critical to ensure that the method of shipping and the time between dispatch and receipt of samples at the lab is well researched.
  3. Clearly indicate the name and telephone number of the person responsible for picking up the package, or receiving it at the laboratory.
  4. Where possible, ship early in the week to avoid delivery during the weekend which may lead to improper storage and loss of samples.
  5. Call the lab first to ensure that the samples are properly collected and packaged and that the samples are expected.
  6. Inform the contact person as soon as the shipment has been sent and provide the name of the carrier, flight number, and waybill number and estimated time of arrival, as appropriate.

Disease Awareness and Control

All samples submitted for disease diagnosis should include as much supporting information as possible including:

  • The contact details of the submitter
  • The reason for submitting the sample (mortalities, abnormal growth, etc.)
  • Handling activities (net/cage de-fouling, size sorting/grading, site changes, predators, new species/ stock introduction, etc.)
  • Relevant Environmental Changes, such as turbidity fluxes, saltwater incursion into freshwater ponds, unusual weather events, etc.

This information will help clarify whether handling stress; change of environment or infectious agents may be a factor in the observed abnormalities/mortalities.

Such information is necessary for both rapid and accurate diagnosis, since it helps focus the investigative procedures required.

If being shipped by air also indicate "HOLD AT AIRPORT AND CALL FOR PICK-UP"

In some cases, samples may be unable to be delivered live to a diagnostic laboratory due to distance or slow transport connections.

In such cases, diagnostic requirements should be discussed with laboratory personnel before you collected your samples. Shipping of non-preserved tissues or dead specimens may require precautions to prevent contamination or decay.

In any event animals that are putrid, or have been dead for any significant length of time, will generally not be suitable for diagnosis.

The laboratory should be informed of the estimated time of arrival of the sample, in order to ensure that the they have all the materials required for processing prepared before the fish arrive.

It is worth noting that many postal services and transport companies (especially air couriers) have strict regulations regarding shipping chemicals, including preserved samples.

If the tissues have been adequately fixed, most fixative or storage solution can be drained from the sample for shipping purposes. As long as sufficient solution is left to keep the tissues from drying out, this will minimise the quantity of chemical solution being shipped.

The carrier should be consulted before samples are collected to ensure they are processed and packed according to shipping rules.

In the case of live samples, clearly indicate the name and telephone number of the person responsible for picking up the package, or receiving it at the laboratory.

Where possible, ship early in the week to avoid delivery during the weekend which may lead to improper storage and loss of samples.

Call the lab first to ensure that the samples are properly collected and packaged and that the samples are expected. Inform the contact person as soon as the shipment has been sent and provide the name of the carrier, flight number, and waybill number and estimated time of arrival, as appropriate.

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WATER SAMPLES

Background

  1. Along with diseased animals, a water sample from the affected pond or growth area should always be submitted to the diagnostic lab.
  2. The volume of water to fish volume/biomass is particularly important for live fish being shipped for ectoparasite examination, so you should check with the diagnostic laboratory before collecting and sending your samples.
  3. The box should be sealed securely to prevent spillage and may be double-packed inside a cardboard carton.
  4. The laboratory should be consulted about the packaging required.
  5. In most States, the local State government representative will be able to advise on the best methods to be used for collecting samples for a particular animals and a particular type of farm.

Disease Awareness and Control
Animal samples should consist of at least 250 ml of water. If in a pond situation the water should be collected from the pond away from the pond edge.

The sample should be collected in a very clean container. Traces of soft drink, juice, detergents, or other chemicals may contaminate sample bottles and lead to inaccurate water test results. It is important to rinse the jar several times in pond water before collecting the sample.
Water samples should be shipped along with the sick animals, but the water in which the fish are shipped is unsuitable for testing.

The water for analysis must be in a bottle separate from the fish. The container with the water sample should be kept cool and out of direct sunlight.

If fish are shipped on ice, put the water sample on ice also.
If samples of plants and algae are to be included, but them in their own container (a bag or bottle as appropriate), don't put them in the water sample. The plants may cause major changes to the water sample during shipping.

A plastic bag measuring 60 x180 cm is suitable for a maximum of four live 200-300 g fish.

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