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ACCURATE DATA
ANIMAL SAMPLES
CONTACTS
TRANSPORT
WATER SAMPLES
ACCURATE DATA
Background
- Record keeping is an essential component of effective
aquatic animal health management.
- These can be included in routine records of an
animal's growth that ideally would be monitored on a regular basis,
either by sub-sampling from tanks or ponds, or by estimates made
from surface observations.
- Where possible, hatcheries should invest in a microscope
and conduct daily microscopic examinations of the larvae.
- This will allow them to quickly identify
problems developing with their stocks, often before they become
evident in the majority of the population.
Disease Awareness and Control
For hatcheries,
critical information that should be recorded include:
- Feeding activity
- Growth (not daily, but regularly)
- Mortalities (number and cause)
- Dissolved oxygen
- Water temperature
These observations should be recorded daily, for all
stages, including date, time, tank number, broodstock (where there
are more than one) and food source.
Dates and times of tank and water changes, pipe flushing/back-flushing
and/or disinfections, should also be recorded.
Ideally, these records should be checked (signed
off) regularly by the person responsible for maintaining the facility.
For pond or
net/cage sites, observations that need to be recorded include:
- Growth
- Fouling
- Mortalities
- Dissolved oxygen
- Water temperature
These should be recorded with date, site location
and any relevant activities (e.g., sample collection for laboratory
examination).
As elsewhere, the person responsible for the facility
should check these records regularly.
The frequency of these observations will vary with site and fish
species.
Human activities should also be recorded on
an "as it happens" basis, since there may be time-lag
effects. In all cases, date and time should be recorded, as parameters
such as temperature and pH can vary markedly during the day, particularly
in open ponds and inter-tidal sites.
All movements of fish into and out of a hatchery or
site should be recorded, including:
- The source of the broodstock/eggs/larvae/juveniles
and their health certification
- The volume or number of fish condition on arrival
- Any quarantine measures carried out
- Date and time of delivery and name of person responsible
for receiving the fish
- Date, time and destination of stock shipped out
from a hatchery or site
Such records are also applicable (but less critical)
to movements between tanks, ponds, and cages within a site.
Where possible, animals from different sources
should not be mixed. If mixing is unavoidable, keep strict records
of which sources are mixed and dates of new introductions into the
holding site or system.
For molluscs, observations
can be included with routine monitoring of mollusk growth, either
by sub-sampling from suspension cages, lines or stakes, or by guess
estimates from surface observations.
Environmental issues must also be monitored on a regular
basis.
This is most applicable to open water sites, but should
also be included in land-based systems with flow-through or well-based
water sources.
The minimum essential data that should be recorded
is:
- Temperature
- Salinity
- Turbidity (qualitative evaluation or secchi disc)
- Algal blooms
and
- Human activity
The frequency of these observations will vary with
site.
Where salinity or turbidity rarely vary, records may
only be required during rainy seasons or exceptional weather conditions.
Temperate climates will require more frequent
water temperature monitoring than tropical climates.
Human activity should be logged on an "as it
happens" basis for reference if no infections or natural environmental
changes can be attributed to a disease situation.
For Crustacean Hatchery
operations, the minimum essential information that should be recorded/
logged includes:
- Feeding activity and feed rates
- Growth/larval staging
- Mortalities
and
- Larval condition
These observations should be recorded on a daily basis
for all stages, and include date, time, tank, broodstock (where
there are more than one) and food-source (e.g., brine shrimp culture
batch or other food-source).
Dates and times for tank and water changes should
also be noted, along with dates and times for pipe flushing and/or
disinfections.
Ideally, the person responsible should check these
logs regularly for the site/animals.
For pond sites, the minimum essential
observations that need to be recorded/logged include:
- Growth
- Feed consumption
- Fouling
and
- Mortality
These should be recorded with date, site location
and any action taken (e.g., sample collection for laboratory examination).
It is important to understand that rates of change
for these parameters are essential for assessing the cause of any
disease outbreak.
This means levels have to be logged on a regular and
consistent basis in order to detect patterns over time.
Ideally, the person responsible for the site/animals
should check these logs regularly.
If an outbreak does occur, the collection of accurate
data that can be passed on to the lab, could mean the difference
between a quick and effective diagnosis; and therefore a remedy;
or a total loss of all animals!
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ANIMAL
SAMPLES
Background
The best time to make observations about farm animals is at a time
when there are no problems on the farm.
"Normal behaviour" of the animals should
be observed from day one to establish and describe the "normal"
situation.
Any change from this normal behaviour should be a
cause for concern and warrants further investigation.
With finfish, abnormal behaviour includes fish swimming
near the surface, sinking to the bottom, loss of balance, flashing,
corkscrewing or air gulping (non air-breathers) or any sign which
deviates from normal behaviour.
Bursts of abnormal activity are often associated with
a generalised lethargy.
If losses persist or increase, samples should be sent
for laboratory analysis.
Mortalities that are unusual, or cannot be explained
should be examined immediately and environmental factors during,
pre- and post-mortality recorded.
Some jurisdictions require farmers to report such
mortalities.
It is difficult to observe behavioural changes in
molluscs in open-water, however, close attention can be made of
behaviour of both broodstock and larvae in hatcheries.
In Crustaceans such as prawns, this may be a subtle
change in feeding behaviour, swimming movement or unusual aggregations.
Even predator activity can provide clues to more "hidden"
changes such as when fish- or shrimp-eating birds congregate round
affected ponds.
Since disease situations can erupt very quickly under
hatchery conditions, regular and close monitoring is essential;
for example, feeding behaviour of larval molluscs is a good indicator
of general health.
Mortalities that spread from one area to another may
suggest the presence of an infectious disease agent and should be
sampled immediately.
Affected animals should be kept (isolated) as far
away as possible from unaffected animals until the cause of the
mortalities can be established.
Disease Awareness and Control
All samples submitted for diagnosis should include as much supporting
information as possible including:
- Contact details of submitter
- Reason(s) for submitting the sample (i.e. health
screening, certification) handling activities
- Gross observations, feed records, and environmental
parameters
- History and origin of the animal population date
of transfer and
- Source location(s) if the stock does not originate
from on-site.
A Contingency Plans will help clarify whether handling
stress, change of environment or infectious agents are causes for
concern.
It will also help speed up diagnosis, risk assessment,
and husbandry management and treatment recommendations.
The most important factors associated with collection
of specimens for surveillance are:
- Sample numbers that are high enough
- Susceptible species are sampled
- Sampling includes age groups and seasons that are
most likely to manifest detectable infections.
Environmental changes (rapid water quality changes,
such as turbidity fluxes, saltwater incursion into freshwater ponds,
unusual weather events, etc.) should also be noted and information
on these events should be included with the specimens.
It's critical to establish, and record, normal behaviour
and appearance to compare with observations made during disease
events.
Accurate record keeping is, therefore, an essential
component of effective aquatic animal health management.
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CONTACTS
Background
- Aquatic environments are complex ecosystems, making
the distinction between health, suboptimal performance and disease
obscure.
- During disease outbreaks, the underlying cause
is often difficult to ascertain and is usually the end result
of a series of linked events involving:
- Environmental factors
- Health condition of the stocks
- Presence of an infectious agent and/or poor husbandry,
and
.
- Management practices.
- The whole aquatic production environment, including
ecological processes, must be taken into consideration when assessing
whether disease is an issue.
- As in pastoral based livestock, the aquaculture
and fisheries sector will continue to face increasing global exposure
to disease agents as it intensifies trade in live aquatic organisms
and their products.
Disease Awareness and Control
The first and most important defenses against
preventable disease losses under such complex situations are:
- Monitoring as regularly as possible and
- Appropriate and decisive action at the first sign
of suspicious behaviour, lesions or mortalities.
Some aquaculture farmers may hesitate to report the
first signs of health problems, due to concern that it may reflect
on their production capability, or that it will result in failure
in the competitive market place. Hiding or denying health problems,
however, can be as destructive to aquatic animals as it is in other
agriculture endeavors.
It is important to recognise that disease is a challenge
that everyone has to face, and having the resources that can effectively
deal with it, are the primary weapons against misplaced ignorance
and fear.
Every State Government has a representative that can
be contacted if an exotic or other disease is suspected.
In Western Australia and in Tasmania the State Governments
provide a diagnostic framework and encourage farmers to make them
the first port of call.
There are also local vets, commercial laboratories
and specialists at many of the TAFE and University based training
Institutions, and of course AFFA from the Australian Government.
The main thing is to act responsibly
and
quickly!
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TRANSPORT
- Shipping samples for diagnosis is as important
as preparing the samples themselves.
- In the case of live animals, it's critical to
ensure that the method of shipping and the time between dispatch
and receipt of samples at the lab is well researched.
- Clearly indicate the name and telephone number
of the person responsible for picking up the package, or receiving
it at the laboratory.
- Where possible, ship early in the week to avoid
delivery during the weekend which may lead to improper storage
and loss of samples.
- Call the lab first to ensure that the samples are
properly collected and packaged and that the samples are expected.
- Inform the contact person as soon as the shipment
has been sent and provide the name of the carrier, flight number,
and waybill number and estimated time of arrival, as appropriate.
Disease Awareness and Control
All samples submitted for disease diagnosis should
include as much supporting information as possible including:
- The contact details of the submitter
- The reason for submitting the sample (mortalities,
abnormal growth, etc.)
- Handling activities (net/cage de-fouling, size
sorting/grading, site changes, predators, new species/ stock introduction,
etc.)
- Relevant Environmental Changes, such as turbidity
fluxes, saltwater incursion into freshwater ponds, unusual weather
events, etc.
This information will help clarify whether handling
stress; change of environment or infectious agents may be a factor
in the observed abnormalities/mortalities.
Such information is necessary for both rapid and accurate
diagnosis, since it helps focus the investigative procedures required.
If being shipped by air also indicate "HOLD AT
AIRPORT AND CALL FOR PICK-UP"
In some cases, samples may be unable to be delivered
live to a diagnostic laboratory due to distance or slow transport
connections.
In such cases, diagnostic requirements should be discussed
with laboratory personnel before you collected your samples. Shipping
of non-preserved tissues or dead specimens may require precautions
to prevent contamination or decay.
In any event animals that are putrid, or have been
dead for any significant length of time, will generally not be suitable
for diagnosis.
The laboratory should be informed of the estimated
time of arrival of the sample, in order to ensure that the they
have all the materials required for processing prepared before the
fish arrive.
It is worth noting that many postal services and
transport companies (especially air couriers) have strict regulations
regarding shipping chemicals, including preserved samples.
If the tissues have been adequately fixed, most fixative
or storage solution can be drained from the sample for shipping
purposes. As long as sufficient solution is left to keep the tissues
from drying out, this will minimise the quantity of chemical solution
being shipped.
The carrier should be consulted before samples are
collected to ensure they are processed and packed according to shipping
rules.
In the case of live samples, clearly indicate the
name and telephone number of the person responsible for picking
up the package, or receiving it at the laboratory.
Where possible, ship early in the week to avoid delivery
during the weekend which may lead to improper storage and loss of
samples.
Call the lab first to ensure that the samples are
properly collected and packaged and that the samples are expected.
Inform the contact person as soon as the shipment has been sent
and provide the name of the carrier, flight number, and waybill
number and estimated time of arrival, as appropriate.
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WATER SAMPLES
Background
- Along with diseased animals, a water sample from
the affected pond or growth area should always be submitted to
the diagnostic lab.
- The volume of water to fish volume/biomass is particularly
important for live fish being shipped for ectoparasite examination,
so you should check with the diagnostic laboratory before collecting
and sending your samples.
- The box should be sealed securely to prevent spillage
and may be double-packed inside a cardboard carton.
- The laboratory should be consulted about the packaging
required.
- In most States, the local State government representative
will be able to advise on the best methods to be used for collecting
samples for a particular animals and a particular type of farm.
Disease Awareness and Control
Animal samples should consist of at least 250 ml of water. If in
a pond situation the water should be collected from the pond away
from the pond edge.
The sample should be collected in a very clean container. Traces
of soft drink, juice, detergents, or other chemicals may contaminate
sample bottles and lead to inaccurate water test results. It is
important to rinse the jar several times in pond water before collecting
the sample.
Water samples should be shipped along with the sick animals, but
the water in which the fish are shipped is unsuitable for testing.
The water for analysis must be in a bottle separate from the fish.
The container with the water sample should be kept cool and out
of direct sunlight.
If fish are shipped on ice, put the water sample on ice also.
If samples of plants and algae are to be included, but them in their
own container (a bag or bottle as appropriate), don't put them in
the water sample. The plants may cause major changes to the water
sample during shipping.
A plastic bag measuring 60 x180 cm is suitable for a maximum of
four live 200-300 g fish.
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